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Ecology
Epidermophyton
is a cosmopolitan dermatophyte, filamentous fungus. Epidermophyton
floccosum is the only species considered as pathogenic as its primary
host is human thus, it is also known as
anthropophilic. Soil is the
natural habitat of the related but the non - pathogenic species
Epidermophyton stockdaleae.
Species
The genus
Epidermophyton contains two species only, namely: Epidermophyton
floccosum and Epidermophyton stockdaleae. Epidermophyton
stockdaleae is known to be non - pathogenic, leaving Epidermophyton
floccosum as the only anthropophilic species causing infections in
humans.
Pathogenicity and Health Effects
Epidermophyton
floccosum is one
of the causative agents of cutaneous infections,
dermatophytosis, in
healthy individuals which particularly infects the skin. Skin infections
include the body surface (tinea corporis),
groin (tinea cruris), feet
(tinea pedis) and nails
(onychomycosis). The fungus lacks
the ability to penetrate the viable tissue of the immunocompetent host
thus, the infection is only restricted to the non – living cornified
layers of epidermis. However, infection due to invasive Epidermophyton
floccosum has been reported in an immunocompromised patient with
Behcet’s syndrome. Furthermore, Epidermophyton floccosum
infections are communicable and can be transmitted by contact,
particularly in common showers and gym facilities.
Macroscopic Appearance
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Growth rate is moderately rapid and colonies mature within 10 days;
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Colonies’ texture is flat, initially grainy and becoming radially grooved,
felty and velvety by maturation and quickly become downy and sterile; and
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Surface colony color is brownish yellow to olive gray or khaki and while
orange to brown on the reverse with an occasional yellow border.
Microscopic Appearance
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Hyaline septate hyphae, macroconidia, and occasionally, chlamydoconidium
– like cells are present while microconidia are typically absent;
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Macroconidia are club – shaped with thin, smooth walls, three to five –
celled, with size of 10 to 40 x 6 to 12 µm, and may be solitary or in
groups; and
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Chlamydoconidium – like cells and
arthroconidia are formed in mature
cultures.
Laboratory Precautions
Only general
laboratory precautions are required, no special safety measures needed.
Susceptibility
In
vitro
antifungal susceptibility testing methods have not been standardized for
Epidermophyton floccosum. However, some reports on in vitro
activity of various antifungal drugs are also available. Most of these
studies have utilized the modifications of the NCCLS M 38P methodology
documented for conidium - forming filamentous fungi. The results of these
analyses show that, ketoconazole, itraconazole, terbinafine, and voriconazole generate low MICs and
appear active in vitro against E. floccosum while
griseofulvin is less active than the other mentioned compounds. On the
other hand, fluconazole generates
the highest MICs and appears to have the lowest activity among the
others. Furthermore, amorolfine
and naftifine also exhibit in
vitro activity against E. floccosum.
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