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Ecology
Trichoderma
is a cosmopolitan, filamentous fungus that is commonly isolated from soil
and from wood. Hypocrea species are the teleomorph of some
Trichoderma species. Trichoderma may cause infections in
presence of certain influential factors, aside from commonly being
considered as a contaminant.
Species
There are five
species belonging to the genus Trichoderma namely, Trichoderma
harzianum, Trichoderma koningii, Trichoderma longibrachiatum,
Trichoderma pseudokoningii, and Trichoderma viride. Apart
from these, there are two other species that have been proposed,
Trichoderma asperelum and Trichoderma citrinoviride; however,
their identity and clinical significance remain unreliable and
unverified. In the differentiation of these
species from one another, the morphological features of their conidia and
phialides are of great importance.
Pathogenicity and Health Effects
Trichoderma
species are
usually considered as non – pathogenic, on the other hand, Trichoderma
viride has been reported as a causative agent of pulmonary infection,
peritonitis in a dialysis patient, and perihepatic infection in a liver
transplant patient. Trichoderma infections are opportunistic in
nature and develop in immunocompromised patients, such as neutropenic
cases and transplant patients, as well as those with chronic renal
failure, chronic lung disease, or amyloidosis. Disseminated infections
due to Trichoderma have also been reported.
Macroscopic Appearance
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Growth rate is rapid and colonies are wooly becoming compact in time; and
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The
surface colony color is white and scattered greenish patches become
visible as the conidia are formed and may form concentric rings at times
while on the reverse, the color is pale, tan, or yellowish;
Microscopic Appearance
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Septate hyaline hyphae, conidiophores, phialides, and conidia are present;
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Trichoderma longibrachiatum
and Trichoderma viride may also produce
chlamydospores;
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Phialides are hyaline, branched, flask – shaped, inflated at the base,
solitary or may appear in clusters, and are attached to the conidiophores
at right angles;
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Conidiophores are hyaline, branched, and may occasionally demonstrate a
pyramidal arrangement; and
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Conidia are unicellular, round or ellipsoidal, green in color, smooth
walled or rough, with an average diameter of 3 µm, and are grouped in
sticky heads at the tips of the phialides, however, these clusters usually
get disrupted during slide preparation procedure intended for microscopic
examination.
Laboratory Precautions
General laboratory
precautions are required, no special safety measures needed.
Susceptibility
Very limited data
are available with regards to the susceptibility activity of
Trichoderma species. MICs of
amphotericin B and
itraconazole may
be elevated. Posaconazole,
caspofungin,
and anidulafungin
show promising activity in vitro against Trichoderma
isolates. To note, voriconazole exerts activity against Trichoderma
longibrachiatum isolates.
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